High-efficiency targeted integration of extrachromosomal arrays in <i>C. elegans</i> using PhiC31 integrase.

ABSTRACT: Extrachromosomal arrays are unique chromosome-like structures created from DNA injected into the C. elegans germline. Arrays are easy to create and allow for high expression of multiple transgenes. They are, however, unstable unless integrated into a chromosome. Current methods for integration, such as X-rays and CRISPR, damage DNA and are low-efficiency. Here, we demonstrate that the viral integrase PhiC31, which mediates a non-mutagenic recombination between short attB and attP sequences, can be used for extremely efficient and targeted integration of arrays. In this method, a transgene, a selectable marker, and attP sites are injected into the gonad of a strain that (1) has an attB site in its genome, and (2) expresses PhiC31 in its germline. F1 extrac

SUBMITTER: Rich MS 

PROVIDER: S-EPMC12642426 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature