Dissecting mammalian cortical circuit development at single-cell resolution using inducible barcoded rabies virus.

ABSTRACT: Highly organized circuits of connected neurons enable diverse brain functions. Improper development of these circuits is associated with neurodevelopmental disorders, and understanding how circuits are formed is crucial for unraveling the mechanisms of these diseases. We currently have an incomplete picture of how specific brain circuits develop and how they are affected in disease, because we lack methods to study them at scale and with single-cell resolution. Monosynaptic rabies tracing is the gold standard method to study circuit architecture. However, it suffers from cellular toxicity, low throughput, lack of control over the timing of labeling, and the inability to access the molecular profiles of individual neurons. To address these issues, we developed an inducible barcoded rabies v

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC12642492 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature