N6-methyladenosine regulation of mRNA translation is essential for early human erythropoiesis.

ABSTRACT: N6-methyladenosine (m⁶A) is an abundant modification of mRNA with important regulatory roles in normal and malignant hematopoiesis. We previously reported that in human erythroid leukemia (HEL) cells, m⁶A mRNA marking selectively regulates translation of essential erythropoiesis genes required for in vitro differentiation and human erythroid colony formation. Here, we further investigated the timing and nature of requirement for m⁶A-methyltransferase (MTase) activity during human erythropoiesis, using a standardized in vitro erythroid differentiation assay for hHSPCs. We identified two critical m⁶A regulated developmental windows in BFU-E and during the transition from CFU-E to proerythroblasts. These windows of m⁶A-MTase requir

SUBMITTER: Kuppers DA 

PROVIDER: S-EPMC12642496 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature