Proximity proteomics reveals the molecular architecture of phytochrome B photobodies in <i>Arabidopsis thaliana</i>.

ABSTRACT: The red/far-red light photoreceptor phytochrome B (phyB) forms light-induced subnuclear condensates, termed photobodies, that coordinate plant responses to light and temperature. Despite their central role in environmental signaling, the molecular composition of phyB photobodies during their early formation has remained unknown. Here, we established an in planta proximity labeling approach using miniTurbo-based biotinylation in Arabidopsis thaliana to capture proteins associated with phyB photobodies during early de-etiolation. Mass spectrometry identified 42 high-confidence proximal proteins, including 11 known core components and 31 previously unrecognized photobody-associated proteins. Among these, the co-chaperone HOP1 forms light-dependent nuclear condensates that partia

SUBMITTER: Olson K 

PROVIDER: S-EPMC12642502 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature