The Lysis Cassette of Jumbophage phiKZ.

ABSTRACT: Pseudomonas jumbophage phiKZ is prominent because of the proteinaceous nuclear structure that is formed during the infection cycle, conferring resistance of the replicating phage DNA to anti-phage resistance systems. Progress has been made in deciphering the molecular basis of nucleus formation and other unique aspects of phiKZ biology, but the understanding of how it causes host lysis remains minimal. Here we present bioinformatic, physiological and molecular evidence for a "lysis cassette", the expression of which is necessary and sufficient for the temporally regulated disruption of the host envelope. This cluster contains genes for the usual components of an MGL (multigene lysis) system, including the holin, endolysin, i-spanin and o-spanin. In addition, a fifth gene in the clus

SUBMITTER: Manohar P 

PROVIDER: S-EPMC12642527 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature