Cell Type Specific Inference of Perturbations in Synaptic Communication with MultiNeuronChat.

ABSTRACT: Inference of differential neuron-neuron and neuron-glia communication from single nucleus RNA sequencing data is a powerful technique to uncover altered communication pathways when comparing groups, such as disease and control, developmental stages, or age groups, or different treatments. Yet, the communication changes are typically not identified on the cell type pair level, limiting its resolution in terms of answering biological and medical questions. Here we present MultiNeuronChat, a highly resolved framework that utilizes gene expression measurements of single cells from case-control single-cell/nucleus RNA-seq data sets together with an existing comprehensive database comprising cell adhesion molecules, gap junctions and synaptic transmission for the differential analysis of neuron-

SUBMITTER: Volkmer G 

PROVIDER: S-EPMC12642661 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature