Ecm19 coordinates mitochondrial fission and cellular redox homeostasis.

ABSTRACT:

Background

Mitochondrial dynamics are tightly coupled with cellular redox homeostasis; however, the underlying regulatory mechanisms remain poorly defined.

Methods

We constructed yeast mutants and evaluated mitochondrial function, morphology, and redox states using growth assays, fluorescence imaging, and flow cytometry. RNA sequencing, RIP assays, and RT-qPCR were applied to identify Ecm19p target genes.

Results

Deletion of ECM19 alone had no evident impact on mitochondrial morphology or respiratory function. In contrast, double deletion of ECM19 with the fusion gene FZO1 (ecm19D fzo1D) rescued mitochondrial function and morphology and reduced ROS and malondialdehyde levels relative to fzo1D. Conversely, combining ecm19D with

SUBMITTER: Chen T 

PROVIDER: S-EPMC12642899 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature