CD8+ T cell depletion promotes human Tph/Tfh cell proliferation and Sjogren syndrome-like symptoms in PBMC-based humanized mice.

ABSTRACT: Peripheral helper T (Tph) and follicular helper T (Tfh) cells are key regulators of B cell differentiation and antibody production, making them promising targets for autoimmune disease treatment. However, their differentiation mechanisms differ significantly between humans and mice, limiting drug validation in mouse models. Here, we present a simple and effective method for in vivo proliferation of human Tph/Tfh and B cells. We discovered that after depleting CD8+ T cells of human peripheral blood mononuclear cell-transferred immunodeficient mice (CD8TΔhPBMC mice), human Tph/Tfh cells and B cells proliferated markedly in the spleen compared with those in human PBMC-transferred immunodeficient mice (hPBMC mice). Transcriptome analysis confirmed proliferating cells' close resemblance to huma

SUBMITTER: Piruzyan M 

PROVIDER: S-EPMC12643515 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature