From wheat bran to equine gut: the <i>in vitro</i> fermentation dynamics of aleurone.

ABSTRACT:

Introduction

Aleurone, a bioactive wheat bran component, has been shown to modulate host metabolism and gut microbiota, but its effects across different compartments of the equine gastrointestinal (GI) tract remain unclear. In this study, we aimed to characterize aleurone-derived metabolite profiles using an in vitro fermentation model with digesta from three equine GI compartments (jejunum, cecum, and colon).

Methods

Three substrates (control feed, aleurone-containing feed, and pure aleurone) were fermented over 72 h, and targeted metabolomics was performed on 38 metabolites.

Results

Significant substrate- and compartment-dependent effects were found for 21 metabolites. Aleurone-containing feed increased asparagine and threonine levels while reducing lactic ac

SUBMITTER: Boshuizen B 

PROVIDER: S-EPMC12643889 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature