Accurate de novo design of high-affinity protein-binding macrocycles using deep learning.

ABSTRACT: Developing macrocyclic binders to therapeutic proteins typically relies on large-scale screening methods that are resource intensive and provide little control over binding mode. Despite progress in protein design, there are currently no robust approaches for de novo design of protein-binding macrocycles. Here we introduce RFpeptides, a denoising diffusion-based pipeline for designing macrocyclic binders against protein targets of interest. We tested 20 or fewer designed macrocycles against each of four diverse proteins and obtained binders with medium to high affinity against all targets. For one of the targets, Rhombotarget A (RbtA), we designed a high-affinity binder (K_d < 10 nM) despite starting from the predicted target structure. X-ray structures for macrocycle-bound myelo

SUBMITTER: Rettie SA 

PROVIDER: S-EPMC12643943 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature