Proximity-based proteomics (BioID) uncovers the Rho GTPase interactome in kidney podocytes.

ABSTRACT:

Introduction

Podocyte injury causes proteinuria. Rho GTPases play critical roles in regulating the podocyte cytoskeleton, and their alteration leads to foot process effacement. Yet, their signaling networks remain poorly understood.

Methodology

To address this, we mapped the interactomes of RhoA, Rac1, and Cdc42 in human podocytes using proximity-dependent biotin identification (BioID) labeling.

Results and discussion

Our BioID analysis detected a total of 1927 interactions with AvgP ≥ 0.95. Approximately 50% of the interactions are unique to podocytes compared to interactions in HEK293 and HeLa cells, with enrichment in pathways related to cell adhesion and shape organization. KIAA1522 emerged as a Rac1/Cdc42 interactor. KIAA1522 knockout reduced cellul

SUBMITTER: Ibrahim S 

PROVIDER: S-EPMC12644026 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature