Optimization of response surface methodology for the extraction of isoliquiritigenin from <i>Aspergillus niger</i> solid-state fermentation of licorice and its antitumor effects.

ABSTRACT: This research focused on optimizing the extraction of isoliquiritigenin (ISL) from licorice via a solid-state fermentation process involving Aspergillus niger. Isoliquiritigenin was quantified through high-performance liquid chromatography (HPLC), initially assessed with one-way analysis and optimized using the Box-Behnken response surface method. The extracted isoliquiritigenin underwent structural modification, and the modified derivatives with enhanced activity were screened for in vivo antitumor efficacy using the MTT Colorimetric (MTT) assay. Finally, the structural and compositional alterations in the intestinal flora of the mice were evaluated post-administration of the extracted isoliquiritigenin. The results indicated that the optimal extraction conditions were pH 3.

SUBMITTER: Hao J 

PROVIDER: S-EPMC12644103 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature