Prognostic value of whole lymph node molecular analysis in colorectal cancer: a prospective multicenter study.

ABSTRACT: Lymph node (LN)-negative colorectal cancer (CRC) patients may be understaged by conventional hematoxylin and eosin for its low sensitivity for detecting LN micrometastasis (LNM). The one-step nucleic acid amplification assay (OSNA) has shown superior performance in detecting LNM. To date, OSNA studies in CRC have analyzed part of the LN tissue with OSNA and part with H&E, introducing a tissue allocation bias for LNM detection. We aimed to evaluate the prognostic significance of the whole LN molecular analysis using OSNA in CRC patients. This prospective multicenter study analyzed LNs from stage I-III CRC by both cytology smears (CS) and OSNA, the latter analyzing the whole LN tissue and reported as the Total Tumor Load (TTL). X-tile software was used to determine the optimal TTL threshold.

SUBMITTER: Liu Q 

PROVIDER: S-EPMC12644202 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature