The TRPV4-YAP axis mediates cytoskeletal and extracellular matrix remodeling in trabecular meshwork cells as a novel glaucoma mechanism.

ABSTRACT: Transient Receptor Potential Vanilloid 4 (TRPV4), a non-selective cation channel in trabecular meshwork (TM) tissue, is activated by forces like deformation and shear stress, playing a critical role in intraocular pressure (IOP) regulation. However, the mechanisms by which TRPV4 mediates TM cells responses to elevated intraocular pressure (IOP), along with the resulting regulatory outcomes, remain incompletely understood. In vitro, TM cells were mechanically stretched, and the effects on TRPV4 activation and calcium influx were evaluated via confocal microscopy, Western blotting, and immunofluorescence. Pharmacological agonists and inhibitors were used to investigate signaling mechanisms. In vivo, ocular hypertension (OHT) was induced in mice, and IOP was measured following drug treatments

SUBMITTER: Xu J 

PROVIDER: S-EPMC12644599 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature