Identification of SARS-CoV-2 3CLpro inhibitors from marine actinomycetes through integrated phylogeny-based metabolomics with functional screening and bioinformatic analysis.

ABSTRACT: As the acute threat of the COVID-19 pandemic diminishes globally, advancing the research and development of antiviral agents remains crucial for ensuring preparedness for future outbreaks. This study investigates the inhibitory effects of marine sediment-derived actinomycetes on the SARS-CoV-2 main protease (3CLpro), a key enzyme involved in viral replication and transcription. Extracts of 75 actinomycete strains from two locations, Nogas Island in Antique and Tubbataha Reefs Natural Park in Palawan, Philippines, were evaluated for their ability to inhibit 3CLpro, with 36% of the extracts showing more than 90% inhibition. Further examination of 27 extracts revealed IC₅₀ values between 2 and 8 µg/mL, suggesting notable inhibitory strength. Phylogenetic analysis grouped the active

SUBMITTER: Dalisay DS 

PROVIDER: S-EPMC12644623 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature