A novel tumor microenvironment-related gene signature for prognostic prediction of intrahepatic cholangiocarcinoma.

ABSTRACT: Intrahepatic cholangiocarcinoma (ICCA) is a highly aggressive bile duct cancer with limited treatment options and poor prognosis. This study aims to develop a gene signature-based model to predict ICCA patient survival. We identified differentially expressed genes (DEGs) from an ICCA mRNA microarray dataset and applied survival, Cox, and LASSO regression analyses to select key genes for constructing the GPSICCA risk score model. The model was then validated in two additional ICCA cohorts, and its relationship with the tumor microenvironment (TME) was analyzed. Additionally, multiplex fluorescent immunohistochemistry (mfIHC) was conducted to examine the expression of key genes in ICCA samples. The expression of COL4A1, GULP1, ITGA6, and STC1 was used to construct the gene set-based prognost

SUBMITTER: Xu D 

PROVIDER: S-EPMC12644728 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature