NF-κB epigenetic attractor landscape drives breast cancer heterogeneity.

ABSTRACT: Heterogeneity in breast cancer (BC) subtypes within a tumor contributes to therapy resistance and cancer recurrence. Subtype heterogeneity in tumors arises through a combination of stochastic genetic/epigenetic changes, phenotypic plasticity, and microenvironment-driven selection as the tumor evolves over time. Here, we sought to characterize how NF-κB epigenetic variability contributes to the progression of the HER2⁺ BC subtype. Initially, we used RNA to determine the expression levels of NF-κB, TWIST1, SIP1, and SLUG in two breast cancer (BC) cell lines, HCC-1954 and MDA-MB-231, classified as HER2⁺ and triple-negative breast cancer (TNBC) subtypes, respectively. Then, we built and calibrated a gene regulatory network (GRN) model that reproduces the transcriptional i

SUBMITTER: Lopes F 

PROVIDER: S-EPMC12644762 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature