Multi-marker analysis of <i>Fasciola gigantica</i> from cattle and buffalo across Pakistan reveals high levels of genetic diversity and novel haplotypes.

ABSTRACT: Molecular analyses of geographically dispersed Fasciola spp. isolates based on ribosomal, mitochondrial and nuclear molecular markers have revealed high levels of genetic diversity within liver fluke populations. To investigate the Fasciola population substructure across Pakistan 4 molecular markers were compared (fatty acid binding protein, fabp; phosphoenolpyruvate carboxykinase, pepck; random amplified polymorphic DNA, RAPD; mitochondrial NADH dehydrogenase, mt-nd1). Adult parasites (n = 595) were collected from buffalo and cattle across 4 provinces in Pakistan (Baluchistan, Gilgit Baltistan, Khyber Pakhtunkhwa, Punjab). Species classification of all 595 parasites was confirmed by the 3 gel-based markers (pepck, fabp and RAPD) as F. gi

SUBMITTER: Komal M 

PROVIDER: S-EPMC12644955 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature