IGF2BP3 regulates EMP1 stability in an m⁶A-dependent manner and activates the TGF-β pathway to promote pancreatic cancer invasion.

ABSTRACT: Local invasion is considered a premonitor of tumor metastasis which cause curative difficulties and undesired prognosis in patients with Pancreatic ductal adenocarcinoma (PDAC). The importance of mRNA N6-methyladenosine (m⁶A) modification during tumor invasion is controversial as it plays distinct roles which mainly attributed to different m⁶A reader proteins exert function. In current study, the level of m⁶A expression in PDAC was analyzed by IHC and ELISA, all m⁶A-regulated genes in PDAC detected by qPCR. The downstream gene EMP1 was screened by analyzing IGF2BP3 knockdown RNA-seq, IGF2BP3-RIP, MeRIP-seq, and PDAC-survival related genes. And m⁶A modification sites of EMP1 RNA was verified by MeRIP-qPCR, RIP-qPCR, and dual luciferase

SUBMITTER: Liu L 

PROVIDER: S-EPMC12645055 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature