Translational Extracellular Matrix Spray-Coating Approach for Large-Scale Bioactive Surface Functionalization and Tissue Repair.

ABSTRACT: While various extracellular matrix (ECM)-based delivery platforms such as cell sheets, nanoparticles, and dressings have demonstrated potential for tissue repair, their clinical translation remains limited by poor scalability, structural fragility, and substrate-specific constraints. This study introduces a translational spray-coating strategy capable of conformationally activating ECM proteins, specifically fibronectin (FN) and collagen (COL), via mechanical unfolding during deposition. Using a commercially available airbrush, micrometer-scale droplets are uniformly deposited onto diverse biomedical substrates, including polydioxanone sutures and oxidized regenerated cellulose/collagen dressings, without structural damage. Notably, FN undergoes conformational changes that enhance cell adh

SUBMITTER: Brilian AI 

PROVIDER: S-EPMC12645076 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature