The Extract of <i>Siegesbeckia orientalis</i> L. Reverses Pro-inflammatory Status of Microglia for Neuroprotection Following Ischemic Stroke in Mice.

ABSTRACT:

Background

Siegesbeckia orientalis L. (SO) is a traditional Chinese herbal medicine commonly used for inflammatory diseases. In ancient, SO is applied for stroke, but mechanisms have not been clear. The purpose is to investigate whether SO exerts neuroprotective effects by reducing microglia-related inflammatory injury after focal cerebral ischemia/reperfusion (I/R).

Methods

SO was extracted using 50% ethanol and quality control was performed by Waters ACQUITY- UPLC CLASS system. The focal cerebral I/R model was established in mice, neurological functions, weight loss and infarct volume was evaluated. Microglial status in penumbra was monitored by immunofluorescence staining and morphology. Mouse primary microglia was subjected to lipopolysaccharide stimulation for t

SUBMITTER: Bowen L 

PROVIDER: S-EPMC12645136 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature