Proteogenomic Discovery of Novel Open Reading Frames With HLA Immune Presentation on Human β-Cells.

ABSTRACT: Ribosome profiling (Ribo-seq) measures translational regulation and reveals novel or unannotated open reading frames (nuORFs) otherwise difficult to identify. Recent reports demonstrate that nuORFs regulate gene expression and immune recognition, highlighting their emerging biological roles. Pancreatic β-cells are critical for maintaining euglycemic conditions, and β-cell impairment contributes to diabetes development. Identification of nuORF and protein/peptide products in human β-cells could reveal novel mechanisms that regulate β-cell function during homeostatic and disease conditions. Here, we applied a proteogenomic approach to human β-cells to define previously unknown protein/peptide products. First, we applied cell type-specific Ribo-seq to map the translatome of human stem cell-de

SUBMITTER: Walters K 

PROVIDER: S-EPMC12645175 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature