Biocontrol of Root-Knot Nematodes via siRNA-Loaded Extracellular Vesicles From a Nematophagous Fungus Arthrobotrys oligospora.

ABSTRACT: Root-knot nematodes (Meloidogyne spp.) represent a major threat to global crop production, and current chemical nematicides pose serious environmental and health risks. RNA interference (RNAi) offers a promising gene-specific strategy for nematode control. However, the efficient and sustainable delivery of RNA molecules into nematodes remains a significant challenge. In this study, we developed an innovative RNA delivery platform using extracellular vesicles (EVs) derived from the nematode-trapping fungus Arthrobotrys oligospora. EVs were either exogenously loaded with synthetic siRNAs targeting the Mi-flp-18 gene of M. incognita or harvested from engineered fungal strains expressing short hairpin RNAs (shRNAs) or double-stranded RNAs (dsRNAs) against multiple nematode neuropeptide genes (

SUBMITTER: Huang X 

PROVIDER: S-EPMC12645230 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature