Precision Molecular Editing: Predicting Substrate Scope and Regiochemistry for CHEESY1, a Flavin Dependent Halogenase.

ABSTRACT: The ability to carry out C-H activation at any site on any heteroaromatic scaffold is a holy grail, offering the potential to revolutionize molecule making. Precision editing, activating, and replacing a C-H bond with a carbon halogen bond opens the way to almost any diversification imaginable. Here, through genome mining and in silico analysis, we present a previously undescribed halogenase tool for precision C-H activation and halogenation. While many halogenated metabolites have been found in the marine environment, indicating the operation of a vast array of halogenases, the presence of such halogenases in other salty environments is less well-known. Here, we describe the first discovery and utilization of a halogenase from a microbe associated with salty and fermented food: spe

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12645471 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature