Using <i>Cupriavidus necator</i> H16 to Provide a Roadmap for Increasing Electroporation Efficiency in Nonmodel Bacteria.

ABSTRACT: Bacteria are a treasure trove of metabolic reactions, but most industrial biotechnology applications rely on a limited set of established host organisms. In contrast, adopting nonmodel bacteria for the production of various chemicals of interest is often hampered by their limited genetic amenability coupled with their low transformation efficiency. In this study, we propose a series of steps that can be taken to increase electroporation efficiency in nonmodel bacteria. As a test strain, we use Cupriavidus necator H16, a lithoautotrophic bacterium that has been engineered to produce a wide range of products from CO₂ and hydrogen. However, its low electroporation efficiency hampers the high-throughput genetic engineering required to develop C. necator into an industr

SUBMITTER: Vajente M 

PROVIDER: S-EPMC12645576 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature