Co-utilization of glucose and xylose in synthetic Escherichia coli consortia for efficient 2-hydroxy-3-methylvalerate biosynthesis.

ABSTRACT: 2-Hydroxy-3-methylvalerate (HMV), a kind of bioactive aliphatic 2-hydroxy acids, is a building block and a key intermediate for pharmaceuticals, including beauvericin, yet its microbial synthesis remains largely unexplored. Here, we present a synthetic biology strategy for high-titer production of HMV that couples mixed-sugar utilization with a division-of-labor microbial consortium. Co-feeding glucose and xylose synchronized substrate uptake with product formation, eliminating intermediate overflow and rerouting carbon from by-products into efficient HMV biosynthesis. To reduce the metabolic burden of the host, the HMV pathway is divided into two engineered strains: one optimized for glucose-to-intermediate conversion, the other for xylose-to-HMV completion. The best consortium, KMV-G-X,

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12645685 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature