Enhanced heterotrophic β-glucan production from a UV-induced Euglena gracilis mutant screened using a novel method under optimized cultivation conditions.

ABSTRACT: The β-glucan produced by Euglena gracilis has emerged as an alternative to traditional high-value biomolecule sources such as yeast and fungi to meet the rising demand. A UV-induced mutant strain, E. gracilis UV199, was developed using a novel aniline-blue-based screening method to increase production. The response surface methodology was used to optimize the growth medium, resulting in glucose and monosodium glutamate concentrations of 25.9 and 5.8 g/L, respectively, and 0.9 × Hutner medium. Using this medium resulted in a β-glucan productivity of 3.62 g/L/day, which was 114% higher than that achieved using the wild type E. gracilis. Productivity was further increased to 4.29 g/L/day through optimizing pH and temperature to 4.5 and 32 °C, respectively. A comprehensive framework was constr

SUBMITTER: Seo H 

PROVIDER: S-EPMC12645731 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature