Carbon source-driven downregulation of the sRNA AmiL enhances <i>Pseudomonas aeruginosa</i> virulence during acute lung infection.

ABSTRACT: Pseudomonas aeruginosa is a major opportunistic pathogen that causes severe acute lung infection. The ability of P. aeruginosa to precisely self-regulate its virulence factors is crucial for enhancing pathogenicity and establishing infection. However, how the bacterium dynamically adjusts virulence gene expression in response to host interaction remains to be elucidated. Here, transcriptome profiling revealed that infection of A549 alveolar epithelial cells significantly upregulated virulence genes associated with the type III secretion system (T3SS) and quorum sensing (QS) in P. aeruginosa PAO1, compared to a standard laboratory growth condition. In contrast, genes of the amiEBCRS operon were markedly downregulated during infection. Notably, AmiL, the leader sm

SUBMITTER: Jing W 

PROVIDER: S-EPMC12645886 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature