The La Crosse virus Gc head domain is a major determinant of infection and pathogenesis.

ABSTRACT: How orthobunyaviruses establish infections and disseminate to cause disease is not well understood. In a previous study using the in vivo evolution of La Crosse virus (LACV), we discovered a cluster of mutations localizing to the LACV Gc head domain. However, we do not understand how the Gc head domain contributes to infection. Here, we generated each of the aforementioned mutations and addressed the role of the Gc head domain in viral replication and infectivity in mammalian and insect cells. We found that specific head domain residues could attenuate replication and infectivity in human neurons and reduce cell binding across different hosts, indicating an important role for the head domain during infection in vitro. Focusing on the in vitro-attenuated Gc N609D varian

SUBMITTER: Dedvukaj A 

PROVIDER: S-EPMC12645916 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature