A HiBiT-tagged pseudovirus-like particle platform for safe, rapid quantification of virus neutralization and antibody-dependent enhancement.

ABSTRACT: Accurate quantification of virus neutralization is essential for evaluating the efficacy of vaccines and antibody-based therapies. However, conventional neutralization assays require strict biocontainment or utilize surrogates of infection that increase biosafety but reduce biological relevance. These limitations create critical bottlenecks for the timely development of antiviral immunotherapeutics. Here, we describe a neutralization platform using non-replicating, HiBiT-tagged pseudovirus-like particles (HiBiT-PsVLPs) for safe yet biologically relevant assessment of neutralization. These HiBiT-PsVLPs package the HiBiT protein tag internally and incorporate glycoproteins from pathogenic viruses to recapitulate entry at reduced biosafety levels. Unlike traditional pseudoviruses, HiBiT-PsVLP

SUBMITTER: Mitchell JK 

PROVIDER: S-EPMC12645950 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature