SARS-CoV-2 polyprotein expression and the induction of double-membrane vesicles.

ABSTRACT: Coronaviruses induce the formation of double-membrane vesicles (DMVs) to facilitate viral RNA replication and transcription by the replication-transcription complexes (RTCs), comprising non-structural proteins (nsps) 2-16 and nucleocapsid protein. Nsp3 and nsp4 are the minimal components necessary for DMV formation and assemble a molecular pore that spans the DMV double membrane, connecting the DMV interior to the cytosol. However, the recruitment mechanisms of additional RTC components and the roles of other viral proteins in DMV assembly remain poorly understood. To dissect these processes independently of viral replication, we sought to establish a surrogate expression system using severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) polypeptides to recapitulate DMV formati

SUBMITTER: Zhao M 

PROVIDER: S-EPMC12645958 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature