Single-base m⁶A epitranscriptomics reveals novel HIV-1 host interaction targets in primary CD4⁺ T cells.

ABSTRACT: N⁶-methyladenosine (m⁶A) plays a critical role in regulating RNA Ostability, localization, and gene expression. m⁶A modification is also important for modulating the expression of viral and cellular genes during HIV-1 infection. However, the function of m⁶A modification in regulating HIV-1 infection of primary CD4⁺ T cells remains unclear. Here, we demonstrate that HIV-1 infection of activated primary CD4⁺ T cells promotes the interaction between the m⁶A writer complex subunits methyltransferase-like 3 and 14 (METTL3/METTL14). Using single-base m⁶A-specific RNA sequencing, we identified differentially m⁶A-modified cellular mRNAs in HIV-1-infected primary CD4⁺ T cells, includ

SUBMITTER: Huang S 

PROVIDER: S-EPMC12645991 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature