Porcine hemagglutinating encephalomyelitis virus VW572 (not Gent/PS412 and Labadie) uses the CD81 receptor and MVB-derived exosomal pathway for efficient entry and spread in neuronal cells.

ABSTRACT: Porcine hemagglutinating encephalomyelitis virus (PHEV) is considered a neurotropic coronavirus that invades the peripheral (PNS) and central (CNS) nervous system of the pig and causes acute encephalomyelitis, also known as "vomiting and wasting disease." Recently, PHEV has been proposed as a potential surrogate virus model to further elucidate the neuropathogenesis of other betacoronaviruses. In this study, we compared key steps in the replication cycle of three distinct PHEV isolates (VW572, Gent/PS412, and Labadie) in mouse neuronal (N2a) cells. We found that PHEV-VW572 replicates more efficiently in these cells compared to the other two isolates. Interestingly, PHEV-VW572 showed high intracellular virus titers without efficient extracellular release. Further investigation revealed that

SUBMITTER: Zaib W 

PROVIDER: S-EPMC12645993 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature