A glycoprotein D-targeted lipid nanoparticle-encapsulated mRNA vaccine elicits strong protective immunity against pseudorabies virus.

ABSTRACT: The pseudorabies virus (PRV), a highly contagious pathogen with zoonotic potential, continues to threaten swine production and public health due to the emergence of virulent variants and insufficient protection conferred by conventional live attenuated vaccines. Although commercial vaccines are safe for pigs, their residual pathogenicity in other susceptible species underscores the demand for universally safe alternatives. Here, we engineered a lipid nanoparticle-encapsulated mRNA vaccine (mRNA-LNPs) expressing PRV glycoprotein D (gD) and evaluated its efficacy in murine and porcine models. In mice, vaccination with gD mRNA-LNPs elicited potent neutralizing antibodies and provided complete protection against lethal PRV challenge. In piglets, immunization induced rapid humoral immune respon

SUBMITTER: Sun Y 

PROVIDER: S-EPMC12645995 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature