A hepatitis B virus RNA-sensing and RNA-editing-dependent reporter system.

ABSTRACT: The compact genomic organization of hepatitis B virus (HBV) has long hindered the development of reporter viruses that do not compromise viral gene expression and replication. Leveraging the advantage of an RNA-sensing and ADAR-editing-dependent cellular reporter system termed reprogrammable adenosine deaminase acting on RNA sensors (RADARS), we developed an HBV-RADARS reporter. In this system, the expression of the reporter gene is activated in trans by HBV RNA-guided, cellular adenosine deaminase acting on RNA 1 (ADAR1)-dependent reporter RNA editing. Using a luciferase reporter, we systematically scanned all ADAR1 targetable sites present in HBV RNAs and selected an optimal sensor sequence. As anticipated, the activation of reporter mRNA

SUBMITTER: Sun L 

PROVIDER: S-EPMC12645999 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature