The neddylation of the RNA-dependent RNA polymerase 3D of Coxsackievirus B3 promotes viral replication.

ABSTRACT: The pathogenesis of Coxsackievirus B3 (CVB3), the causative pathogen for severe diseases such as myocarditis, pancreatitis, and meningitis, remains largely unknown. Neddylation, the covalent modification of proteins through the conjugation of NEDD8, a ubiquitin-like molecule, plays critical roles in regulating cellular activities. Our previous study demonstrated that 3D^pol, the RNA-dependent RNA polymerase of CVB3, was modified by ubiquitin. Since NEDD8 is highly homologous to ubiquitin in both the sequence and structure, we hypothesized that 3D^pol may also undergo neddylation. Here, we demonstrated that 3D^pol of CVB3 is modified by NEDD8. Proteomics and immunoprecipitation studies identified that NEDD8 expression was upregulated in CVB3-infected cells, and

SUBMITTER: Li S 

PROVIDER: S-EPMC12646006 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature