Comparative analysis of NSP5/VP2-induced viroplasm-like structures in rotavirus species A to J.

ABSTRACT: Rotavirus (RV) is classified into nine species, A-D and F-J, with RV species A (RVA) being the most extensively studied. While RVA infects infants and young animals, non-RVA species infect adult humans, various mammals, and birds. However, the lack of appropriate research tools has limited our understanding of non-RVA life cycles. RVA replication and assembly occur in cytosolic inclusions termed viroplasms. We recently identified viroplasm-like structures (VLS) composed of NSP5 and NSP2 in non-RVA. In this context, globular VLS induced by NSP2 formed in RVA, RVB, RVD, RVF, RVG, and RVI, but not in RVC, RVH, and RVJ. Additionally, in RVA, VLS can also be formed through the co-expression of NSP5 with VP2. Here, we report that VP2-induced VLS formed in RV species A to J, with notable formatio

SUBMITTER: Cosic A 

PROVIDER: S-EPMC12646009 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature