A scalable human gut-immune co-culture model for evaluating inflammatory bowel disease anti-inflammatory therapies.

ABSTRACT: Current treatments for inflammatory bowel disease (IBD) are often ineffective long-term, as many patients ultimately become unresponsive to anti-inflammatory drugs. The need for improved therapeutics is urgent. Animal models utilized for drug development are limited by interspecies variability and poor translatability. However, most in vitro models lack the sophistication to model the key interplay of the immune system with the intestinal epithelium in line with the known role of the immune system in the etiology of the disease. To address this gap, we developed a primary intestinal epithelial cell co-culture system to incorporate elements of innate immune signaling. This system models immune-epithelial interactions using RepliGut^Ⓡ - Planar Transverse Colon cultured on a Transwe

SUBMITTER: Peddibhotla S 

PROVIDER: S-EPMC12646107 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature