Fibroblastic reticular cells form reactive myeloid cell niches in human lymph nodes.

ABSTRACT: Lymph nodes play a key role in maintaining fluid balance in homeostatic and inflamed tissues and provide fibroblastic niche environments for optimal immune cell positioning and interaction. Here, we used single-cell and spatial transcriptomic analyses in combination with high-resolution imaging to molecularly define and functionally characterize niche-forming cells that control inflammation-driven remodeling in human lymph nodes. Fibroblastic reticular cells responded to inflammatory perturbation with activation and expansion of poised niche environments. Inflammation-induced adaptation of lymph node infrastructure and topography included the expansion of peptidase inhibitor 16 (PI16)-expressing reticular cell (PI16⁺ RC) networks that enwrap the perivenular conduit system. Inter

SUBMITTER: Lutge M 

PROVIDER: S-EPMC12646167 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature