Contribution of particle-induced lysosome membrane permeabilization to NLRP3 inflammasome activation and mitochondrial ROS production.

ABSTRACT: Nickel oxide nanoparticles (NiONPs) can trigger reactive oxygen species (ROS) production and NLRP3 inflammasome activation in macrophages as key steps in promoting lung inflammation. However, the impact of NiONP-induced lysosome membrane permeabilization (LMP) on mitochondrial ROS (mtROS) production and NLRP3 inflammasome activation is unknown. Murine ex vivo alveolar macrophages (mexAM) were exposed to NiONPs or crystalline silica (cSiO2) as a positive control particle. Imipramine was used to inhibit LMP before measuring mtROS and NLRP3 inflammasome activation and MitoTEMPO was used to block mtROS before measuring LMP and NLRP3 inflammasome activation. The ability of phagocytosed NiONPs to leach Ni2+ and stimulate ROS production was also assessed. Exposure to either particle resulted in L

SUBMITTER: Albright JM 

PROVIDER: S-EPMC12646592 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature