Comprehensive Phenotypic and Proteomic Analyses of Human Reticulocyte Maturation.

ABSTRACT: Reticulocyte maturation, the final stage of erythropoiesis following enucleation, is a dynamic 48- to 72-hour process remodeling multi-lobular, motile, and fragile nascent reticulocytes into deformable, biconcave red blood cells. This maturation involves organelle clearance and extensive membrane remodeling. A significant knowledge gap in our understanding is a lack of knowledge of the proteins involved in the earliest stages of reticulocyte maturation, the nascent bone marrow reticulocyte. To address this, we isolated and characterized the proteomic profiles of distinct reticulocyte populations from healthy human bone marrow. Density gradient separation, FACS analyses, and electron microscopy revealed a continuum of different stages of reticulocyte maturation in the marrow. Deformation-in

SUBMITTER: Leduc M 

PROVIDER: S-EPMC12646609 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature