Evaluation of serological assays for <i>intra vitam</i> diagnosis of bovine tuberculosis in water buffalo (<i>Bubalus bubalis</i>).

ABSTRACT:

Introduction

Bovine tuberculosis (TB) in water buffalo (Bubalus bubalis) is primarily diagnosed using intra vitam tests, as the intradermal tuberculin test (IDT) and the interferon-gamma release assay (IGRA), both of which detect cell-mediated immunity (CMI). However, a subset of infected animals fails to mount a detectable CMI response, posing a significant risk of undetected transmission. Serological tests assessing the humoral immune response could provide a valuable complementary tool for identifying infected animals that escape detection through traditional CMI-based assays.

Methods

This study analyzed 895 serum samples from water buffaloes, including 393 from TB-free herds and 502 from TB-infected herds. Animals from TB-free herds were tested using IDT an

SUBMITTER: Giovannozzi S 

PROVIDER: S-EPMC12646987 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature