Identification of ferroptosis-genes associated with pediatric inflammatory bowel disease bioinformatics and machine learning approaches.

ABSTRACT:

Background

Pediatric inflammatory bowel disease (PIBD) is increasingly common, and early diagnosis remains challenging due to unclear etiology. Ferroptosis, an iron-dependent form of cell death, may be involved in intestinal inflammation, but its expression and role in PIBD are poorly understood.

Objective

To identify ferroptosis-related genes as candidate biomarkers for early diagnosis of PIBD and validate their role in ferroptosis.

Methods

RNA-seq data of PIBD from GEO datasets were analyzed using DESeq2, WGCNA, and functional enrichment analysis. Ferroptosis-related diagnostic genes were screened through LASSO, Random Forest, and mSVM-RFE algorithms, and validated in GSE57945 and GSE117993 datasets. In vitro experiments using NCM460 cells were performed to v

SUBMITTER: Xu Z 

PROVIDER: S-EPMC12647037 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature