Qingfei Dayuan granules and decoction alleviate acute lung injury via TLR4 signaling pathway modulation, gut microbiota regulation, and metabolic reprogramming.

ABSTRACT:

Objective

To explore and compare the therapeutic effects and underlying mechanisms of Qingfei Dayuan Granules (QFDYGs) and Qingfei Dayuan Decoctions (QFDYDs) for the treatment of acute lung injury (ALI), focusing on the modulation of the TLR4 signaling pathway, intestinal microbiota, and related metabolic pathways.

Methods

The active metabolite contents were measured by ultrahigh-performance liquid chromatography. A mouse model of lipopolysaccharide-induced ALI (tracheal instillation) was used to assess efficacy. After drug administration, lung tissue damage was analyzed by hematoxylin-eosin staining and quantification of inflammatory cytokines and oxidative stress biomarkers with enzyme-linked immunosorbent assays. Components of the TLR4 signaling pathway were quantified by

SUBMITTER: Cheng H 

PROVIDER: S-EPMC12647075 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature