Integrated computational and experimental approach to identify Nrf2-regulated molecular targets in cerebral ischemia.

ABSTRACT:

Background

The transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) is a master regulator of the cellular antioxidant response, playing an important role in protecting neurons from ischemic injury. The hippocampus exhibits region-specific vulnerability to ischemia, with CA1 neurons being highly susceptible, while CA2-3 and dentate gyrus (DG) neurons demonstrate greater resistance. Our previous work revealed higher basal and post-ischemia/reperfusion (I/R) Nrf2 activity in the resistant CA2-3,DG region compared to CA1. This study aimed to identify potential Nrf2-regulated genes that contribute to this regional neuroprotection in a gerbil model of global cerebral ischemia.

Methods

We used a combined computational and experimental approach. By utilizing the m

SUBMITTER: Lewczuk A 

PROVIDER: S-EPMC12647287 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature