Metabolic enzyme PFKFB3 mediates matrix stiffness-potentiated tumour growth and radiotherapeutic resistance in HCC.

ABSTRACT:

Background

Although the contribution of matrix stiffness to aggravating the malignant features of HCC cells has been well documented, the effects of matrix stiffness on chemoradiotherapy resistance and its underlying mechanism remain largely elusive.

Methods

To delineate the role of matrix stiffness in HCC progression, we engineered novel in vivo animal models with defined liver stiffness and a complementary tunable hydrogel culture system. This integrated approach enabled a comprehensive investigation into how biomechanical cues modulate HCC cell proliferation and DNA repair both in vitro and in vivo.

Results

High stiffness stimulation noticeably enhanced cell proliferation and cell survival from DNA damage through changing the expression and distribution of metaboli

SUBMITTER: Wang M 

PROVIDER: S-EPMC12647364 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature