Targeted mutagenesis of <i>FATTY ACID ELONGASE 1</i> entails near complete elimination of very long chain fatty acids in the seeds of camelina cultivar Ligena.

ABSTRACT: Genome editing has the potential to enhance yield and quality traits of crops. However, standard genetic transformation methods are not always applicable to modern germplasm. To tackle this challenge in the widely cultivated variety Ligena of the oilseed crop camelina (Camelina sativa (L.) Crantz), an only recently established principle of adventitious shoot formation from immature zygotic embryos was employed to further improve its fatty acid profile. In this approach, the three subgenomic homeologs of the FATTY ACID ELONGASE 1 (FAE1) gene were subjected to targeted mutagenesis. To pre-validate the Cas9-interacting, target motif-specific guide (g)RNAs, a robust protoplast-based DNA transfection method was established. This assay demonstrated that the preselected gRNAs

SUBMITTER: Rezaeva BR 

PROVIDER: S-EPMC12647405 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature