The RING-domain E3 ubiquitin ligase OsRGLG6 regulates rice grain number and yield via ubiquitination-mediated degradation of OsOTUB1.

ABSTRACT: Rice (Oryza sativa) yield is a complex trait determined by multiple factors, including grain number per panicle (GPP), which is mainly genetically regulated. In this study, we identified the E3 RING DOMAIN LIGASE (RGLG) OsRGLG6 as a key regulator of GPP in rice. A yeast two-hybrid screen revealed that OsRGLG6 interacts with OsOTUB1, an ovarian tumor domain-containing ubiquitin-specific protease involved in panicle development. Further experiments confirmed the interaction between OsRGLG6 and OsOTUB1 in vitro and in vivo. OsRGLG6, which contains a vWA domain and a RING domain, is localized in the cytoplasm and endoplasmic reticulum and is highly expressed specifically in rice panicles. In vitro and in vivo ubiquitination assays demonstrated that OsRGLG6 ubiquitinates OsOTUB1 and prom

SUBMITTER: Chen J 

PROVIDER: S-EPMC12647413 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature