The peanut <i>Ubiquitin4</i> promoter drives stable gene overexpression and efficient multiplex CRISPR/Cas9 gene editing in peanut.

ABSTRACT: The development of effective genetic transformation tools is crucial for advancing molecular breeding in peanut (Arachis hypogaea). In this study, we identified and characterized a Ubiquitin (UBQ) promoter from peanut and evaluated its potential utility in transgenic research. Using sequence similarity-based identification and transcriptome analysis, we selected a highly expressed UBQ gene, arahy.E356RC, designated AhUBQ4, from which we cloned a 973-bp fragment of the promoter region. To assess its activity, we used this AhUBQ4 promoter fragment to drive expression of the GUS and Ruby reporter genes in transient and stable expression assays in Nicotiana benthamiana and peanut tissues. Compared to the commonly used CaMV 35S prom

SUBMITTER: Cui Y 

PROVIDER: S-EPMC12647447 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature