Profiling of main regulators of angiogenesis and inflammation in human PBMC and plasma revealed associations with lower extremity artery disease.

ABSTRACT: Lower extremity artery disease (LEAD) represents the most common atherosclerosis-related disease in the group of peripheral artery diseases. A growing amount of evidence suggests that dysregulations in signaling pathways associated with angiogenesis and inflammation can contribute to the progression and onset of LEAD; however, this association is still poorly understood. Therefore, in our study, we investigated expression levels of 18 genes that encode key regulators of angiogenesis and inflammation, as well as plasma levels of their protein products, in patients with LEAD in comparison with non-LEAD controls. Gene expression levels were determined in peripheral blood mononuclear cells (PBMC) using real-time PCR, and protein plasma levels were measured using ELISA. The expression levels of

SUBMITTER: Zalewski D 

PROVIDER: S-EPMC12647588 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature